Difference between revisions of "Part:BBa K823052"
 
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lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside).  
 
lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside).  
  
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[[Image:LacZ plate.png|thumb|right|400px|lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.]]
 
This BioBrick was used for promoter evaluation in ''B. subtilis''.  
 
This BioBrick was used for promoter evaluation in ''B. subtilis''.  
  
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We used this ''lacZ'' gene to evaluate our promoters [https://parts.igem.org/Part:BBa_K823000 PliaG], [https://parts.igem.org/Part:BBa_K823001 PliaI], [https://parts.igem.org/Part:BBa_K823003 Pveg].
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We used this ''lacZ'' gene to evaluate our promoters [https://parts.igem.org/Part:BBa_K823000 PliaG], [https://parts.igem.org/Part:BBa_K823001 PliaI], [https://parts.igem.org/Part:BBa_K823003 Pveg] in [https://parts.igem.org/Part:BBa_K823022 pSB<sub>''Bs''</sub>4S] with [https://static.igem.org/mediawiki/parts/7/70/LMU-Munich_2012_%C3%9F-Galactosidase_Assay_B._subtilis.pdf β-galactosidase-assays for ''B. subtilis'']. This gene also works in ''E. coli'' which was qualitively seen by blue colonies on LB-Agar-plates that contain X-Gal.
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[[Image:LacZ plate.png|thumb|center|400px|lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.]]
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Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks ''<b>Bacillus'' B</b>io<b>B</b>rick <b>B</b>ox]
  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 12:17, 26 September 2012

lacZ, B. subtilis optimized + RBS + Term

lacZ in Freiburg standard, Bacillus subtilis codon optimized with ribosome binding site and terminator (B0014).

lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).

lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.

This BioBrick was used for promoter evaluation in B. subtilis.

Find out more about the design of our prefix with ribosome binding site.

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT


We used this lacZ gene to evaluate our promoters PliaG, PliaI, Pveg in pSBBs4S with β-galactosidase-assays for B. subtilis. This gene also works in E. coli which was qualitively seen by blue colonies on LB-Agar-plates that contain X-Gal.

Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks Bacillus BioBrick Box]









Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]