Difference between revisions of "Part:BBa K864403"
 
(One intermediate revision by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K864403 short</partinfo>
 
<partinfo>BBa_K864403 short</partinfo>
  
−
This part contains <partinfo>BBa_J23110</partinfo>, <partinfo>BBa_B0034</partinfo> and <partinfo>BBa_K864401</partinfo>.
+
This part is a coding device for the chromoprotein aeBlue (<partinfo>BBa_K864401</partinfo>) by means of promoter <partinfo>BBa_J23110</partinfo> and the RBS <partinfo>BBa_B0034</partinfo>.
−
 
+
−
aeBlue (<partinfo>BBa_K864401</partinfo>) is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
+
−
 
+
−
 
+
  
 +
aeBlue (more info on <partinfo>BBa_K864401</partinfo>) is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
  
  
Line 18: Line 15:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
This part is useful for visual screening and for example be used instead of can be used as
  
 
<!-- -->
 
<!-- -->

Latest revision as of 23:57, 25 September 2012

J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)

This part is a coding device for the chromoprotein aeBlue (BBa_K864401) by means of promoter BBa_J23110 and the RBS BBa_B0034.

aeBlue (more info on BBa_K864401) is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.


IMG 4371a.jpg AeBlue onplate.jpg

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]