Difference between revisions of "Part:BBa K819017"
 
(2 intermediate revisions by 2 users not shown)
Line 5: Line 5:
 
SulA promoter belongs to the SOS regulon family. In <i>E.coli</i> it promotes the transcription of SulA protein. LexA protein in <i>E.coli</i> can bind to SulA promoter, inhibiting the transcription of the genes downstream.<br />
 
SulA promoter belongs to the SOS regulon family. In <i>E.coli</i> it promotes the transcription of SulA protein. LexA protein in <i>E.coli</i> can bind to SulA promoter, inhibiting the transcription of the genes downstream.<br />
 
However, with mutations in specific sites which LexA protein will bind to, SulA408 promoter will not be bound by LexA protein, but is compatible to LexA408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.<br />
 
However, with mutations in specific sites which LexA protein will bind to, SulA408 promoter will not be bound by LexA protein, but is compatible to LexA408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.<br />
−
For example, the optimized luminesensor with a LexA408 domain ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819005 BBa_K819005]) constructed by [http://2012.igem.org/Team:Peking Peking 2012 iGEM team] can specifically bind to SulA408 promoter and therefore inhibit the gene expression (e.g. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819006 BBa_K819006] is a testing device containing SulA408 promoter constructed by Peking 2012 iGEM team)<br /><br />
+
For example, the optimized <i>Luminesensor</i> with a LexA408 domain ([https://parts.igem.org/Part:BBa_K819005 BBa_K819005]) constructed by [http://2012.igem.org/Team:Peking Peking 2012 iGEM team] can specifically bind to SulA408 promoter and therefore inhibit the gene expression (e.g. [https://parts.igem.org/Part:BBa_K819006 BBa_K819006] is a testing device containing SulA408 promoter constructed by Peking 2012 iGEM team)<br /><br />
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 15: Line 15:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
 
−
===Functional Parameters===
+
 
 +
==Functional Parameters: Austin_UTexas==
 +
<html>
 +
<body>
 
<partinfo>BBa_K819017 parameters</partinfo>
 
<partinfo>BBa_K819017 parameters</partinfo>
−
<!-- -->
+
<h3><center>Burden Imposed by this Part:</center></h3>
 +
<figure>
 +
<div class = "center">
 +
<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
 +
</div>
 +
<figcaption><center><b>Burden Value: 2.7 ± 3.2% </b></center></figcaption>
 +
</figure>
 +
<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
 +
<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
 +
<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
 +
</body>
 +
</html>

Latest revision as of 18:50, 3 September 2020

Luminesensor repressible SulA408 promoter

SulA promoter in 408 form.
SulA promoter belongs to the SOS regulon family. In E.coli it promotes the transcription of SulA protein. LexA protein in E.coli can bind to SulA promoter, inhibiting the transcription of the genes downstream.
However, with mutations in specific sites which LexA protein will bind to, SulA408 promoter will not be bound by LexA protein, but is compatible to LexA408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.
For example, the optimized Luminesensor with a LexA408 domain (BBa_K819005) constructed by [http://2012.igem.org/Team:Peking Peking 2012 iGEM team] can specifically bind to SulA408 promoter and therefore inhibit the gene expression (e.g. BBa_K819006 is a testing device containing SulA408 promoter constructed by Peking 2012 iGEM team)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_K819017 parameters

Burden Imposed by this Part:

Burden Value: 2.7 ± 3.2%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.