Difference between revisions of "Part:BBa K801065:Design"

Latest revision as of 11:29, 16 October 2012

(+)-limonene synthase 1 with Strep-Tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1676
Illegal SapI.rc site found at 100

C-Terminal Strep-Tag added

See BBa_K801061

Keywords: limonen, strep-tag

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

BioBrick encodes (+)-limonene synthase
UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:
cytotoxic for S. cervisiae growth inhibition at 1 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

BioBrick: BBa_I742111

Organism:

Gene sequence derived from Citrus limon

References

Literature references:

[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71]

Database references:

[http://www.ncbi.nlm.nih.gov/nucleotide/21435702 GenBank: Citrus limon (+)-limonene synthase 1 mRNA, complete cds]
[http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]

@@ Line 13: / Line 13: @@
 <br>'''Keywords:'''
 <!--These keywords are necessary to find your part using a fulltext sarch.-->
-<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
+limonen, strep-tag
 <br>'''Abbreviations:'''
@@ Line 22: / Line 22: @@
 '''Related BioBrick:'''
+* [https://parts.igem.org/Part:BBa_K801060 BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801061 BBa_K801061: (+)-Limonene synthase 1 coding region]
+* [https://parts.igem.org/Part:BBa_K801062 BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast]
+* [https://parts.igem.org/Part:BBa_K801063 BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast]
+* [https://parts.igem.org/Part:BBa_K801064 BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast]
+* [https://parts.igem.org/Part:BBa_K801066 BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression]
 <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
@@ Line 36: / Line 42: @@
 '''Backbone:'''<br>
-<!--*Backbone name: pSB1C3'/?backbone_name?-->
+* Backbone name: pSB1C3
-<!--*Resistance: Amp/Cp/Kan/Tet-->
+* Resistance: Cp
-<!--*Copynumber: low/medium/high-->
+* Copynumber: high
 '''Protein coding:'''<br>
-<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+* BioBrick encodes (+)-limonene synthase
-<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
+* UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
-<!--*The protein encoded is posttranslationally modified by ???.-->
+<!--none/not known/cytotoxic for ''organism name''-->
-<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 '''Enzymatic activity:'''
-<!--none/EC-number ?.?.?.?-->
+* E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
 '''Cytotoxicity:'''<br>
-<!--none/not known/cytotoxic for ''organism name''-->
+cytotoxic for ''S. cervisiae'' growth inhibition at 1 mM, lethal effect at 100 mM
 '''Safety notes:'''<br>
@@ Line 66: / Line 70: @@
 '''Source:'''<br>
+*BioBrick: BBa_I742111
 <!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
@@ Line 76: / Line 81: @@
 '''Organism:'''<br>
+* Gene sequence derived from ''Citrus limon''
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===
@@ Line 86: / Line 91: @@
 '''Literature references:'''<br>
-<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
+*[http://www.ncbi.nlm.nih.gov/pubmed/12084056 '''Pubmed:''' Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71]
 '''Database references:'''<br>
-<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
+*[http://www.ncbi.nlm.nih.gov/nucleotide/21435702 '''GenBank''': Citrus limon (+)-limonene synthase 1 mRNA, complete cds]
 <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
-<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
+*[http://www.uniprot.org/uniprot/Q8L5K3 '''Uniprot''': (R)-limonene synthase 1]
 <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 <!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->