Difference between revisions of "Part:BBa K784046:Design"
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<partinfo>BBa_K784046 short</partinfo> | <partinfo>BBa_K784046 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | The riboswitch | + | The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(N4) RNAP are adjacent to one another without any restriction site between them. |
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===Source=== | ===Source=== | ||
− | + | Assembly PCR of [https://parts.igem.org/Part:BBa_K784005 BBa K784005] and [https://parts.igem.org/Part:BBa_K784044 K784044] | |
===References=== | ===References=== |
Latest revision as of 10:39, 26 September 2012
theophylline riboswitch +T7*(N4) RNAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 15
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(N4) RNAP are adjacent to one another without any restriction site between them.
Source
Assembly PCR of BBa K784005 and K784044