Difference between revisions of "Part:BBa K864403"

(Added part BBa_K864403 pSB1K3 Promoter+RBS+aeBlue)
 
 
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aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. aeBlue is of the GFP family with significant sequence similarities.
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__NOTOC__
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<partinfo>BBa_K864403 short</partinfo>
  
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The protein was discovered, extracted and analyzed in (Shkrob, 2005)
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This part is a coding device for the chromoprotein aeBlue (<partinfo>BBa_K864401</partinfo>) by means of promoter <partinfo>BBa_J23110</partinfo> and the RBS <partinfo>BBa_B0034</partinfo>.
  
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aeBlue (more info on <partinfo>BBa_K864401</partinfo>) is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
  
  
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[[Image:IMG 4371a.jpg|400px]]
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[[Image:AeBlue_onplate.jpg|400px]]
  
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Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.
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===References===
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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This part is useful for visual screening and for example be used instead of can be used as
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K864403 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K864403 parameters</partinfo>
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<!-- -->

Latest revision as of 23:57, 25 September 2012

J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)

This part is a coding device for the chromoprotein aeBlue (BBa_K864401) by means of promoter BBa_J23110 and the RBS BBa_B0034.

aeBlue (more info on BBa_K864401) is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.


IMG 4371a.jpg AeBlue onplate.jpg

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]