Difference between revisions of "Part:BBa K907001"
 
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[[Image: KAIST_DNA_recombination_via_Bxb1_Int,_Xis.png |center|450px]]
 
[[Image: KAIST_DNA_recombination_via_Bxb1_Int,_Xis.png |center|450px]]
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This part is a protein coding part which encodes '''DNA excisionase''' of ''Mycobacterium phage Bxb1''.
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This part is a protein coding part which encodes Bxb1 gp47, which is a '''DNA excisionase''' of ''Mycobacterium phage Bxb1''.<br>
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The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called ''attB'' and ''attP'', and then integrates, inverts, or excises dsDNA depending on the orientation of recognition sequences.
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The Bxb1 gp47 is a protein that directly binds to integrase-DNA complex. Without gp47, integrase-DNA complex recognize the "attL" and "attR" very weakly. The complex with gp47 strengthen the recognition and catalyzes DNA recombination reaction which is exactly reverse one done by integrase alone.
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We used this integrase to invert specific sequence in plasmid. This protein is well expressed in ''E.coli(strain MG1655)''.
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When it inverts DNA, the ''attB'' and ''attP'' sequences are changed into'' attL'' and ''attR'', as other DNA recombinases do. Another protein called Bxb1 excisionase forms complex with integrase and this complex flips inverted DNA back into original sequence by regenerating ''attB'' and ''attP'' sequences.  
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'''<Related Parts>'''
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907000] - Mycobacterium Phage Bxb1 integrase
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907002] - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907003] - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907004] - Dual Phase Protein Generator(GFP default). mRFP and GFP
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907005] - Dual Phase Protein Generator(mRFP default). mRFP and GFP
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 12:15, 26 September 2012

Mycobacterium Phage Bxb1 gp47, DNA excisionase


KAIST DNA recombination via Bxb1 Int, Xis.png

This part is a protein coding part which encodes Bxb1 gp47, which is a DNA excisionase of Mycobacterium phage Bxb1.
The Bxb1 gp47 is a protein that directly binds to integrase-DNA complex. Without gp47, integrase-DNA complex recognize the "attL" and "attR" very weakly. The complex with gp47 strengthen the recognition and catalyzes DNA recombination reaction which is exactly reverse one done by integrase alone.

<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]