Difference between revisions of "Part:BBa K801065:Design"

 
(→‎Design Notes)
 
(8 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801065 short</partinfo>
 
<partinfo>BBa_K801065 short</partinfo>
Line 6: Line 5:
  
  
−
===Design Notes===
+
 
 
C-Terminal Strep-Tag added
 
C-Terminal Strep-Tag added
  
  
 +
See BBa_K801061
 +
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 +
limonen, strep-tag
 +
 +
<br>'''Abbreviations:'''
 +
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
 +
<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
 +
 +
===Design Notes===
 +
 +
'''Related BioBrick:'''
 +
* [https://parts.igem.org/Part:BBa_K801060 BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801061 BBa_K801061: (+)-Limonene synthase 1 coding region]
 +
* [https://parts.igem.org/Part:BBa_K801062 BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast]
 +
* [https://parts.igem.org/Part:BBa_K801063 BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast]
 +
* [https://parts.igem.org/Part:BBa_K801064 BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast]
 +
* [https://parts.igem.org/Part:BBa_K801066 BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression]
 +
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 +
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 +
 +
'''Cloning details:'''<br>
 +
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 +
<!--*Mutation C889G to delete XbaI restriction site-->
 +
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 +
 +
'''Quality control measures:'''<br>
 +
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 +
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 +
<!--*Part was partly sequenced/Part was totally sequenced-->
 +
 +
'''Backbone:'''<br>
 +
* Backbone name: pSB1C3
 +
* Resistance: Cp
 +
* Copynumber: high
 +
 +
'''Protein coding:'''<br>
 +
* BioBrick encodes (+)-limonene synthase
 +
* UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
 +
<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Enzymatic activity:'''
 +
* E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
 +
'''Cytotoxicity:'''<br>
 +
cytotoxic for ''S. cervisiae'' growth inhibition at 1 mM, lethal effect at 100 mM
 +
 +
'''Safety notes:'''<br>
 +
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
 +
 +
'''Intellectual property:'''
 +
<!--Information on patent situation.-->
 +
<!--Intellectual property claims made by the authors.-->
 +
 +
'''Corresponding part author/authors:'''
 +
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
−
See BBa_K801061
+
'''Source:'''<br>
 +
*BioBrick: BBa_I742111
 +
<!--*Commercial system: plasmid name, system name, company name-->
 +
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 +
<!--*Preexisting BioBrick ?Bba_number?-->
 +
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 +
<!--*Synthesized by ?company_name?.-->
 +
 
 +
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
 
 +
'''Organism:'''<br>
 +
* Gene sequence derived from ''Citrus limon''
 +
<!--*Genesequence derived from ''?organism_name?''-->
 +
<!--*Codonoptimized for ''?organism_name?''-->
 +
<!--*Designed for the following Chassis: ''?organism-name?''-->
 +
<!--*Statement about functionality in other chassis.-->
  
 
===References===
 
===References===
 +
<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
*[http://www.ncbi.nlm.nih.gov/pubmed/12084056 '''Pubmed:''' Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71]
 +
 +
'''Database references:'''<br>
 +
*[http://www.ncbi.nlm.nih.gov/nucleotide/21435702 '''GenBank''': Citrus limon (+)-limonene synthase 1 mRNA, complete cds]
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 +
*[http://www.uniprot.org/uniprot/Q8L5K3 '''Uniprot''': (R)-limonene synthase 1]
 +
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 +
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 +
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 11:29, 16 October 2012

(+)-limonene synthase 1 with Strep-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1676
    Illegal SapI.rc site found at 100


C-Terminal Strep-Tag added


See BBa_K801061


Keywords: limonen, strep-tag


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • BioBrick encodes (+)-limonene synthase
  • UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

  • E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:
cytotoxic for S. cervisiae growth inhibition at 1 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • BioBrick: BBa_I742111


Organism:

  • Gene sequence derived from Citrus limon

References

Literature references:

  • [http://www.ncbi.nlm.nih.gov/pubmed/12084056 Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71]

Database references:

  • [http://www.ncbi.nlm.nih.gov/nucleotide/21435702 GenBank: Citrus limon (+)-limonene synthase 1 mRNA, complete cds]
  • [http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]