Difference between revisions of "Part:BBa K818100"
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− | This a promoter from B. subtilis which is upregulated when in the presence of the volatiles from rotten meat. For more information, visit iGEM Groningen 2012 page. | + | This a promoter from ''B. subtilis'' which is upregulated when in the presence of the volatiles from rotten meat. For more information, visit iGEM Groningen 2012 page. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | We connected the SboA promoter to different pigment genes (chromoproteins AmilCP([[Part:BBa_K592009|BBa_K592009]]) & AmilGFP([[Part:BBa_K592010|BBa_K592010]]), and lycopene ([[Part:BBa_K274100|BBa_K274100]]). You can find a detailed characterization of the expression of SboA in the page of the SboA-AGFP device ([[Part:BBa_K818600|BBa_K818600]], or check out the [https://static.igem.org/mediawiki/parts/5/50/RR_Groningen_818600_Data_sheet.pdf data sheet]). | ||
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Latest revision as of 03:40, 27 October 2012
Promoter sboA, upregulated by rotten meat volatiles in B. subtilis
This a promoter from B. subtilis which is upregulated when in the presence of the volatiles from rotten meat. For more information, visit iGEM Groningen 2012 page.
Usage and Biology
We connected the SboA promoter to different pigment genes (chromoproteins AmilCP(BBa_K592009) & AmilGFP(BBa_K592010), and lycopene (BBa_K274100). You can find a detailed characterization of the expression of SboA in the page of the SboA-AGFP device (BBa_K818600, or check out the data sheet).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]