Difference between revisions of "Part:BBa K907002"
 
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<partinfo>BBa_K907002 short</partinfo>
 
<partinfo>BBa_K907002 short</partinfo>
  
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Dual phase protein generator, RBS(reverse) - attB - Promoter - attP - RBS
 
  
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[[Image: KAIST_BBa_K907002.png |300px]]
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[[Image: KAIST_BBa_K907002.png |300px]]
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[[Image: KAIST_BBa_K907002.png |300px]]
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<br>This part is composed of 3 elements.
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'''<Part Description>'''
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<br>1. RBS: BBa_B0034, upsteam one is reversed
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[[Image: KAIST_BBa_K907002.png |center|600px]]
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<br>2. Promoter: BBa_J23119, Bacterial constitutive promoter
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<br> This part is composed of 3 elements.
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<br>3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase
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<br>     1. RBSs: [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], upsteam one is reversed
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We designed this part to generate two different proteins one at a time.
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<br>     2. Promoter: [https://parts.igem.org/Part:BBa_J23119 BBa_J23119], Bacterial constitutive promoter
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<br>     3. att sites: Recognition site for [https://parts.igem.org/Part:BBa_K907000 BBa_K907000](Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase).
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'''<Binary Signal Generation>'''
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<br>This part promotes transcription and translation of two different proteins one at a time when work with [https://parts.igem.org/Part:BBa_K907000 BBa_K907000] and [https://parts.igem.org/Part:BBa_K907001 BBa_K907001]. By doing so,  this device can generate two different signals or binary signal. This binary signal can be further used in variety of modules or even in biological computing in the future.
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'''<Applications>'''
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[[Image: KAIST_Dual_Phase_protein_generator_default.png |center|600px]]
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<br>[[Image: KAIST_Dual_Phase_protein_generator_inverted.png |center|600px]]
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Initially, this device promotes the transcription and translation of downstream gene due to its promoter orientation. When Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase, recognizes and inverts the sequence flanking with attB and attP sequences, promoter orientation is inverted. Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step).
  
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At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation.
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When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).
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'''<Part Demonstration>'''
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<br>This device is tested by GFP and mRFP attached construct. See [https://parts.igem.org/Part:BBa_K907004 BBa_K907004].
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This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N
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'''<Related Parts>'''
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907000] - Mycobacterium Phage Bxb1 integrase
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907001] - Mycobacterium Phage Bxb1 excisionase
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907003] - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907004] - Dual Phase Protein Generator(GFP default). mRFP and GFP
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<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907005] - Dual Phase Protein Generator(mRFP default). mRFP and GFP
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:16, 26 September 2012

Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS


<Part Description>

KAIST BBa K907002.png


This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att sites: Recognition site for BBa_K907000(Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase).

<Binary Signal Generation>
This part promotes transcription and translation of two different proteins one at a time when work with BBa_K907000 and BBa_K907001. By doing so, this device can generate two different signals or binary signal. This binary signal can be further used in variety of modules or even in biological computing in the future.

<Applications>

KAIST Dual Phase protein generator default.png

KAIST Dual Phase protein generator inverted.png

Initially, this device promotes the transcription and translation of downstream gene due to its promoter orientation. When Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase, recognizes and inverts the sequence flanking with attB and attP sequences, promoter orientation is inverted. Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step).

<Part Demonstration>
This device is tested by GFP and mRFP attached construct. See BBa_K907004.

<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907001 - Mycobacterium Phage Bxb1 excisionase
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 75
    Illegal NheI site found at 98
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 42
    Illegal BsaI.rc site found at 125