Difference between revisions of "Part:BBa K896008:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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[[Image:flu 200.jpg]]
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*Before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au.
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[[Image:Cd 15.jpg]]
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*This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion.  
  
 
===Applications of BBa_K896008===
 
===Applications of BBa_K896008===
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It can be used to detect cadmium ion when ligating with GFP.
  
 
===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K896008 AddReview number</partinfo>
 
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<I>Username</I>
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<I>iGEM Nagahama_2014</I>
 
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Enter the review inofrmation here.
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lac promoter ([https://parts.igem.org/Part:BBa_R0010 BBa_R0010]) in the upper of the zinTp was removed from BBa_K896008 resulting to new parts ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342005 BBa_K1342005]).
 
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Latest revision as of 15:00, 17 October 2014


Flu 200.jpg

  • Before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au.

Cd 15.jpg

  • This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion.

Applications of BBa_K896008

It can be used to detect cadmium ion when ligating with GFP.

User Reviews

UNIQd05b02d262d8dad3-partinfo-00000000-QINU


No review score entered. iGEM Nagahama_2014

lac promoter (BBa_R0010) in the upper of the zinTp was removed from BBa_K896008 resulting to new parts (BBa_K1342005).

;

UNIQd05b02d262d8dad3-partinfo-00000002-QINU