Difference between revisions of "Part:BBa K784018"
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br> | This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br> | ||
| − | Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the | + | Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome. <br> |
| + | More information is available [http://2012.igem.org/Team:Technion/Project/Phage here]. <br> | ||
Fragment 4 contains the phages tail proteins, which together with fragment 1 [[Part:BBa_K784015| (BBa_K784015)]], 2 [[Part:BBa_K784016| (BBa_K784016)]], and 3 [[Part:BBa_K784017| (Part:BBa_K784017)]] create the phage envelope. <br> | Fragment 4 contains the phages tail proteins, which together with fragment 1 [[Part:BBa_K784015| (BBa_K784015)]], 2 [[Part:BBa_K784016| (BBa_K784016)]], and 3 [[Part:BBa_K784017| (Part:BBa_K784017)]] create the phage envelope. <br> | ||
Latest revision as of 19:31, 26 September 2012
Phage Lambda fragment 4 - tail fiber proteins
This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 4 contains the phages tail proteins, which together with fragment 1 (BBa_K784015), 2 (BBa_K784016), and 3 (Part:BBa_K784017) create the phage envelope.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1747
Illegal EcoRI site found at 6625
Illegal XbaI site found at 5029
Illegal PstI site found at 354
Illegal PstI site found at 802
Illegal PstI site found at 2942
Illegal PstI site found at 7449 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1747
Illegal EcoRI site found at 6625
Illegal PstI site found at 354
Illegal PstI site found at 802
Illegal PstI site found at 2942
Illegal PstI site found at 7449 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1747
Illegal EcoRI site found at 6625
Illegal BglII site found at 2946
Illegal BamHI site found at 2867 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1747
Illegal EcoRI site found at 6625
Illegal XbaI site found at 5029
Illegal PstI site found at 354
Illegal PstI site found at 802
Illegal PstI site found at 2942
Illegal PstI site found at 7449 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1747
Illegal EcoRI site found at 6625
Illegal XbaI site found at 5029
Illegal PstI site found at 354
Illegal PstI site found at 802
Illegal PstI site found at 2942
Illegal PstI site found at 7449
Illegal NgoMIV site found at 561
Illegal AgeI site found at 144
Illegal AgeI site found at 215
Illegal AgeI site found at 2599 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 7755
Illegal SapI.rc site found at 5290