Difference between revisions of "Part:BBa K784017"
 
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
 
This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
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Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome. <br>
+
Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome. <br>
 +
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here]. <br>
 
Fragment 3 contains the phages tail tip proteins, which together with fragment 1 [[Part:BBa_K784015| (BBa_K784015)]], 2 [[Part:BBa_K784016| (BBa_K784016)]], and 4 [[Part:BBa_K784018| (BBa_K784018)]] create the phage envelope. <br>
 
Fragment 3 contains the phages tail tip proteins, which together with fragment 1 [[Part:BBa_K784015| (BBa_K784015)]], 2 [[Part:BBa_K784016| (BBa_K784016)]], and 4 [[Part:BBa_K784018| (BBa_K784018)]] create the phage envelope. <br>
  

Latest revision as of 19:24, 26 September 2012

Phage Lambda fragment 3 - tail tip proteins

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 3 contains the phages tail tip proteins, which together with fragment 1 (BBa_K784015), 2 (BBa_K784016), and 4 (BBa_K784018) create the phage envelope.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1365
    Illegal PstI site found at 1452
    Illegal PstI site found at 3152
    Illegal PstI site found at 3302
    Illegal PstI site found at 4461
    Illegal PstI site found at 6904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1365
    Illegal PstI site found at 1452
    Illegal PstI site found at 3152
    Illegal PstI site found at 3302
    Illegal PstI site found at 4461
    Illegal PstI site found at 6904
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1365
    Illegal PstI site found at 1452
    Illegal PstI site found at 3152
    Illegal PstI site found at 3302
    Illegal PstI site found at 4461
    Illegal PstI site found at 6904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1365
    Illegal PstI site found at 1452
    Illegal PstI site found at 3152
    Illegal PstI site found at 3302
    Illegal PstI site found at 4461
    Illegal PstI site found at 6904
    Illegal AgeI site found at 4046
    Illegal AgeI site found at 6183
    Illegal AgeI site found at 6362
    Illegal AgeI site found at 6694
    Illegal AgeI site found at 6765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 357