Difference between revisions of "Part:BBa K863122"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K863122 short</partinfo> | <partinfo>BBa_K863122 short</partinfo> | ||
− | [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] | + | GFP with constant promoter and His-tag made from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] (without the terminators). |
+ | Originally designed to have a comparison to a His-tagged GFP to cellulose binding domain fusion protein. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | GFP (mut3b) [note that this part does not have a barcode] | ||
+ | |||
+ | [[Help:Tag|Tag]]ged version of gfp from Repressilator reporter. See the [[Part:BBa_E0040:Design | design page]] for more source information. | ||
+ | |||
+ | <!-- | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_E0040 SequenceAndFeatures</partinfo> | ||
+ | <!-- --> | ||
+ | <!-- | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_E0040 parameters</partinfo> | ||
+ | <!-- --> | ||
+ | |||
+ | ====Fluorescence wavelengths==== | ||
+ | Cormack ''et al''.<cite>Cormack</cite> report the following excitation and emission data for GFPmut3 - <br> | ||
+ | *'''Excitation max''' - 501nm | ||
+ | *'''Emission max''' - 511nm | ||
+ | |||
+ | ====Latency==== | ||
+ | Cormack ''et al''.<cite>Cormack</cite> report detectable fluorescence within 8 mins. | ||
+ | |||
+ | ==Pictures== | ||
+ | <gallery> | ||
+ | Image:I13522 lightbox.jpg|BBa_I13522 visualized under non-UV lightbox | ||
+ | Image:I13522 - UV 254nm.JPG|BBa_I13522 visualized under 254nm wavelength UV lightbox | ||
+ | </gallery> | ||
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Latest revision as of 22:03, 26 September 2012
BBa_I13522 with His-tag
GFP with constant promoter and His-tag made from BBa_I13522 (without the terminators). Originally designed to have a comparison to a His-tagged GFP to cellulose binding domain fusion protein.
Usage and Biology
GFP (mut3b) [note that this part does not have a barcode]
Tagged version of gfp from Repressilator reporter. See the design page for more source information.
Fluorescence wavelengths
Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -
- Excitation max - 501nm
- Emission max - 511nm
Latency
Cormack et al.Cormack report detectable fluorescence within 8 mins.
Pictures
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 813
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724