Difference between revisions of "Part:BBa K907001"

 
 
(4 intermediate revisions by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K907001 short</partinfo>
 
<partinfo>BBa_K907001 short</partinfo>
  
−
This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1.
+
----
−
The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences.
+
<br>
−
We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655).
+
[[Image: KAIST_DNA_recombination_via_Bxb1_Int,_Xis.png |center|450px]]
−
When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.  
+
This part is a protein coding part which encodes Bxb1 gp47, which is a '''DNA excisionase''' of ''Mycobacterium phage Bxb1''.<br>
 +
The Bxb1 gp47 is a protein that directly binds to integrase-DNA complex. Without gp47, integrase-DNA complex recognize the "attL" and "attR" very weakly. The complex with gp47 strengthen the recognition and catalyzes DNA recombination reaction which is exactly reverse one done by integrase alone.
  
 +
----
 +
'''<Related Parts>'''
 +
<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907000] - Mycobacterium Phage Bxb1 integrase
 +
<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907002] - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
 +
<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907003] - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
 +
<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907004] - Dual Phase Protein Generator(GFP default). mRFP and GFP
 +
<br>[https://parts.igem.org/Part:BBa_K907001 BBa_K907005] - Dual Phase Protein Generator(mRFP default). mRFP and GFP
 +
----
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 12:15, 26 September 2012

Mycobacterium Phage Bxb1 gp47, DNA excisionase


KAIST DNA recombination via Bxb1 Int, Xis.png

This part is a protein coding part which encodes Bxb1 gp47, which is a DNA excisionase of Mycobacterium phage Bxb1.
The Bxb1 gp47 is a protein that directly binds to integrase-DNA complex. Without gp47, integrase-DNA complex recognize the "attL" and "attR" very weakly. The complex with gp47 strengthen the recognition and catalyzes DNA recombination reaction which is exactly reverse one done by integrase alone.

<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]