Difference between revisions of "Part:BBa K731701:Design"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K731701 short</partinfo>
 
<partinfo>BBa_K731701 short</partinfo>
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This is the [[Part:BBa_K731700|BBa_K731700]] platform with the [[Part:BBa_K731721|BBa_K731721]] T7 terminator inserted in the BioBrick cloning region. This part was used in [[Part:BBa_K731721|BBa_K731721]] characterization.
 
  
 
<partinfo>BBa_K731701 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K731701 SequenceAndFeatures</partinfo>

Latest revision as of 10:56, 19 September 2012

BBa_K731700 with T7terminator (BBa_K731721)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6850
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6850
    Illegal NheI site found at 6879
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6850
    Illegal BglII site found at 6011
    Illegal BamHI site found at 6832
    Illegal XhoI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6850
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6850
    Illegal XbaI site found at 6865
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 714
    Illegal NgoMIV site found at 1051
    Illegal NgoMIV site found at 4231
    Illegal NgoMIV site found at 4391
    Illegal NgoMIV site found at 5979
    Illegal AgeI site found at 6800
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2228
    Illegal SapI.rc site found at 3310


Design Notes

The measurements with this plasmid were made using the E. coli strain BL21(DE3)pLysS. This is a lysogen strain that expresses T7 RNA polymerase. The expression in this strain give a very low basal expression.

Source

This is a composite part made of BBa_K731721 inserted in the BBa_K731700 backbone. The backbone is derived from the RL024A, a plasmid made by Roberta Lentini and kindly provided to the Trento iGEM team by the Mansy lab. The terminator has been extracted by PCR from pET21b vector.

References