Difference between revisions of "Part:BBa K777117"
 
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This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and  are required for the flow of ions and torque generation.
 
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and  are required for the flow of ions and torque generation.
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<br><br>
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IISER_BHOPAL 2021
 
===Usage and Biology===
 
===Usage and Biology===
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It is an amphipathic protein with 308 long amino acid residues, having 22 residues long hydrophobic region near N-terminus. Reports have shown that membranes have limited capacity to hold this protein, and insertion of MotB in membrane requires other components, including MotA protein.
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===References===
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Stader, J., Matsumura, P., Vacante, D., Dean, G. and Macnab, R., 1986. Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. Journal of Bacteriology, 166(1), pp.244-252.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K777117 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K777117 SequenceAndFeatures</partinfo>
 
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[[Image:K777117_in_pSB1C3.jpg|frame|left|'''Fig. 1:''' Plasmid map of K777117 in pSB1C3]]
 
[[Image:K777117_in_pSB1C3.jpg|frame|left|'''Fig. 1:''' Plasmid map of K777117 in pSB1C3]]
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==Functional Parameters: Austin_UTexas==
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===Functional Parameters===
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<html>
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<body>
 
<partinfo>BBa_K777117 parameters</partinfo>
 
<partinfo>BBa_K777117 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: -2.4 ± 2.4% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 14:52, 9 October 2021

motB

This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.

IISER_BHOPAL 2021

Usage and Biology

It is an amphipathic protein with 308 long amino acid residues, having 22 residues long hydrophobic region near N-terminus. Reports have shown that membranes have limited capacity to hold this protein, and insertion of MotB in membrane requires other components, including MotA protein.

References

Stader, J., Matsumura, P., Vacante, D., Dean, G. and Macnab, R., 1986. Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. Journal of Bacteriology, 166(1), pp.244-252.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 310


Fig. 1: Plasmid map of K777117 in pSB1C3



Functional Parameters: Austin_UTexas

BBa_K777117 parameters

Burden Imposed by this Part:

Burden Value: -2.4 ± 2.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.