Difference between revisions of "Part:BBa K823019"
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This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS. | This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS. | ||
+ | lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside). | ||
+ | [[Image:LacZ plate.png|thumb|right|400px|lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.]] | ||
+ | This BioBrick was used for promoter evaluation in ''B. subtilis''. | ||
+ | |||
+ | Find out more about the design of our [https://static.igem.org/mediawiki/parts/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf prefix with ribosome binding site]. | ||
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | ||
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+ | We used this ''lacZ'' gene to evaluate our promoters [https://parts.igem.org/Part:BBa_K823000 PliaG], [https://parts.igem.org/Part:BBa_K823001 PliaI], [https://parts.igem.org/Part:BBa_K823003 Pveg] in [https://parts.igem.org/Part:BBa_K823022 pSB<sub>''Bs''</sub>4S] with [https://static.igem.org/mediawiki/parts/7/70/LMU-Munich_2012_%C3%9F-Galactosidase_Assay_B._subtilis.pdf β-galactosidase-assays for ''B. subtilis'']. This gene also works in ''E. coli'' which was qualitively seen by blue colonies on LB-Agar-plates that contain X-Gal. | ||
+ | Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks ''<b>Bacillus'' B</b>io<b>B</b>rick <b>B</b>ox] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 12:17, 26 September 2012
LacZ (RBS+Freiburg standard)
This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the Freiburg standard with RBS.
lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).
This BioBrick was used for promoter evaluation in B. subtilis.
Find out more about the design of our prefix with ribosome binding site.
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
We used this lacZ gene to evaluate our promoters PliaG, PliaI, Pveg in pSBBs4S with β-galactosidase-assays for B. subtilis. This gene also works in E. coli which was qualitively seen by blue colonies on LB-Agar-plates that contain X-Gal.
Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks Bacillus BioBrick Box]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]