Difference between revisions of "Part:BBa K823030:Experience"
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These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] | These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] | ||
| − | [[Image:Activity PcotYZ PcotV.png|thumb| | + | [[Image:Activity PcotYZ PcotV.png|thumb|center|400px]] |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 14:15, 7 September 2012
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K823030
We used the promtoer PcotYZ for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.
This part was used to display fusion proteins on B. subtilis spores.
These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033
User Reviews
UNIQd65dfd9c0982eb72-partinfo-00000000-QINU UNIQd65dfd9c0982eb72-partinfo-00000001-QINU