Difference between revisions of "Part:BBa K624003"
 
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<partinfo>BBa_K624003 short</partinfo>
 
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Only the plasmid contain rep gene can be used in magnetic bacteria AMB-1. This gene will express suficient protein for plasmid DNA replication in AMB-1.
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Only the plasmid contain rep gene can be used in magnetic bacteria AMB-1. Rep gene will express suficient protein for plasmid DNA replication in AMB-1.
  
 
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[[image:Rep.jpg|500px|]]
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===Functional Parameters===
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<partinfo>BBa_K624003 parameters</partinfo>
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[[image:Rep.jpg|500px|]]
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
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<partinfo>BBa_K624003 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: -0.9 ± 6.8% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</html>

Latest revision as of 03:48, 25 August 2020

rep gene

Only the plasmid contain rep gene can be used in magnetic bacteria AMB-1. Rep gene will express suficient protein for plasmid DNA replication in AMB-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 613
  • 1000
    COMPATIBLE WITH RFC[1000]


Rep.jpg



Functional Parameters: Austin_UTexas

BBa_K624003 parameters

Burden Imposed by this Part:

Burden Value: -0.9 ± 6.8%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.