Difference between revisions of "Part:BBa K539392"

Latest revision as of 06:50, 8 October 2011

Vio operon ABDE(each preceded by their own ribosome binding site) with a terminator

Built in vioABCE (actually is vioABDE Part:BBa_K274004) and a terminator (Part:BBa_J61048).

We found out that there's a point mutation in the BBa_K274004 . This mutation occurs in the Xba I restriction site which takes a lot of time for us just to digest it in order to ligase this part with our interest part.

We also notice something that this part is actually vioABDE instead of vioABCE.

In previous time, we failed in every attempts to clone out vioC from this part. We then decided to do DNA sequencing to make sure every single sequence is correct. Afterward, we found the exact sequence of vioABDE instead of vioABCE.

The vioABDE catalyze L-Tryptophane to protoviolaceinic acid and produce a dark green thing. It can be added any part you want to improvise its fuction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1029
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3814
Illegal NgoMIV site found at 6040
Illegal AgeI site found at 4010
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 5239
Illegal SapI.rc site found at 5314

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K539392 short</partinfo>
-Built in vioABCE (actually vioABDE [[Part:BBa_K274004]]) and a terminator ([[Part:BBa_J61048]]).
+Built in vioABCE (actually is vioABDE [[Part:BBa_K274004]]) and a terminator ([[Part:BBa_J61048]]).
 We found out that there's a point mutation in the [https://parts.igem.org/Part:BBa_K274004:Experience  BBa_K274004] . This mutation occurs in the Xba I restriction site which takes a lot of time for us just to digest it in order to ligase this part with our interest part.