Difference between revisions of "Part:BBa K574009"
 
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<partinfo>BBa_K574009 short</partinfo>
 
<partinfo>BBa_K574009 short</partinfo>
  
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A transcription factor, LasR (''<partinfo>C0079</partinfo>'') that is active in the presence of cell-cell signaling molecule 3OC12HSL is controlled by a constitutive regulated promoter (''<partinfo>BBa_J23100</partinfo>''). Device input is 3OC12HSL. Device output is [[PoPS]] from a LasR-regulated operator.  
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This composite part can be considered as 3OC12HSL in -> PoPS out device, because 3OC12HSL binds LasR (''<partinfo>C0079</partinfo>'') which turns on pLas promoter(''<partinfo>R0079</partinfo>''). This device produces lasR constitutively, under the control of a constitutive regulated promoter (''<partinfo>BBa_J23100</partinfo>''). Device input is 3OC12HSL. Device output is [[PoPS]] from the Plas promoter(''<partinfo>R0079</partinfo>'').
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[[Image:k574009_chart.png]]
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We ligate this part with E0840 (''<partinfo>E0840</partinfo>''), and measure the intensity of GFP in cultures with different concentrations of 3OC12HSL.
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From the chart, we can see that cells were hardly induced in the control group, and with the concentration of inducer growing, the intensity of GFP increased by groups. The most efficient concentration of inducer was around 10^-5M, and higher concentration may lead to the expression of GFP decreasing. Additionally, to most groups, the intensity of GFP reached its maxium after 4 hours.
  
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Reference:''<partinfo>F2620</partinfo>''
 
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 01:41, 6 October 2011

3OC12HSL -> PoPS Receiver

This composite part can be considered as 3OC12HSL in -> PoPS out device, because 3OC12HSL binds LasR (BBa_C0079) which turns on pLas promoter(BBa_R0079). This device produces lasR constitutively, under the control of a constitutive regulated promoter (BBa_J23100). Device input is 3OC12HSL. Device output is PoPS from the Plas promoter(BBa_R0079).

K574009 chart.png

We ligate this part with E0840 (BBa_E0840), and measure the intensity of GFP in cultures with different concentrations of 3OC12HSL.

From the chart, we can see that cells were hardly induced in the control group, and with the concentration of inducer growing, the intensity of GFP increased by groups. The most efficient concentration of inducer was around 10^-5M, and higher concentration may lead to the expression of GFP decreasing. Additionally, to most groups, the intensity of GFP reached its maxium after 4 hours.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1048
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]