Difference between revisions of "Part:BBa J52008:Experience"

Latest revision as of 13:31, 30 September 2011

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Please enter how you used this part and how it worked out.

Applications of BBa_J52008

User Reviews

UNIQ38bfd6af3d5eabc6-partinfo-00000000-QINU UNIQ38bfd6af3d5eabc6-partinfo-00000001-QINU

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iGEM TUDelft 2008 Ostassen

During our project of constructing an RNA thermometer to repress RNA translation we've used this part succesfully to produce luciferase. For more info check [http://2008.igem.org/Team:TUDelft/Temperature_results#Luciferase_Measurements this link]. Graphs visible over there are luciferase activity measurements.

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Catramp

The Utah_State 2011 iGEM Team has confirmed that this part emits light when placed under control of a promoter and the appropriate luciferin is added, and is capable of working with standard luciferase testing kits and luminometers.

Improvements: This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier (Dual Luciferase Assay Component).

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UT-Tokyo

UT-Tokyo 2011 Team has characterized that this part emits luminescence with a promoter and substrate (D-luciferin). For testing this device, we carried out a promoter assay using BBa_R0011 and BBa_J23119 as a control. We succesfully evaluated the relative expression levels of BBa_R0011 with various IPTG concentration, compared to that of BBa_J23119. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].

Improvements: UT-Tokyo 2011 team has utilized this part to devise a Firefly-Renilla Dual Luciferase Assay Kit. This kit makes an evaluation of promoters and other cis-elements much easyer and more quantitative than existing devices (including GFP-reporter system). We also conducted a calibration of Photinus pyralis luciferase using a standard reagent, demonstrating that intracellular luciferase amount can be directly estimated from relative luminescence unit (RPU) by a linear regression. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].

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UNIQ38bfd6af3d5eabc6-partinfo-00000005-QINU

@@ Line 35: / Line 35: @@
 '''Improvements: '''This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier ([https://parts.igem.org/Part:BBa_K654045 Dual Luciferase Assay Component]).
-|}
+|};
+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<partinfo>BBa_I712019 AddReview 5</partinfo>
+<I>UT-Tokyo</I>
+|width='60%' valign='top'|
+UT-Tokyo 2011 Team has characterized that this part emits luminescence with a promoter and substrate (D-luciferin).
+For testing this device, we carried out a promoter assay using [https://parts.igem.org/Part:BBa_R0011 BBa_R0011] and [https://parts.igem.org/Part:BBa_J23119 BBa_J23119] as a control. We succesfully evaluated the relative expression levels of [https://parts.igem.org/Part:BBa_R0011 BBa_R0011] with various IPTG concentration, compared to that of [https://parts.igem.org/Part:BBa_J23119 BBa_J23119]. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].
+'''Improvements:'''
+UT-Tokyo 2011 team has utilized this part to devise a [https://parts.igem.org/Part:BBa_K518002 Firefly-Renilla Dual Luciferase Assay Kit]. This kit makes an evaluation of promoters and other cis-elements much easyer and more quantitative than existing devices (including GFP-reporter system).
+We also conducted a calibration of Photinus pyralis luciferase using a standard reagent, demonstrating that intracellular luciferase amount can be directly estimated from relative luminescence unit (RPU) by a linear regression. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].
+|};
 <!-- DON'T DELETE --><partinfo>BBa_J52008 EndReviews</partinfo>