Difference between revisions of "Part:BBa J52008:Experience"

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The Utah_State 2011 iGEM Team has confirmed that this part emits light when placed under control of a promoter and the appropriate luciferin is added, and is capable of working with standard luciferase testing kits and luminometers.
 
The Utah_State 2011 iGEM Team has confirmed that this part emits light when placed under control of a promoter and the appropriate luciferin is added, and is capable of working with standard luciferase testing kits and luminometers.
  
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This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier ([https://parts.igem.org/Part:BBa_K654045 Dual Luciferase Assay Component]).  
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'''Improvements: '''This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier ([https://parts.igem.org/Part:BBa_K654045 Dual Luciferase Assay Component]).  
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<partinfo>BBa_I712019 AddReview 5</partinfo>
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<I>UT-Tokyo</I>
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UT-Tokyo 2011 Team has characterized that this part emits luminescence with a promoter and substrate (D-luciferin).
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For testing this device, we carried out a promoter assay using [https://parts.igem.org/Part:BBa_R0011 BBa_R0011] and [https://parts.igem.org/Part:BBa_J23119 BBa_J23119] as a control. We succesfully evaluated the relative expression levels of [https://parts.igem.org/Part:BBa_R0011 BBa_R0011] with various IPTG concentration, compared to that of [https://parts.igem.org/Part:BBa_J23119 BBa_J23119]. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].
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'''Improvements:'''
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UT-Tokyo 2011 team has utilized this part to devise a [https://parts.igem.org/Part:BBa_K518002 Firefly-Renilla Dual Luciferase Assay Kit]. This kit makes an evaluation of promoters and other cis-elements much easyer and more quantitative than existing devices (including GFP-reporter system).
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We also conducted a calibration of Photinus pyralis luciferase using a standard reagent, demonstrating that intracellular luciferase amount can be directly estimated from relative luminescence unit (RPU) by a linear regression. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].
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<!-- DON'T DELETE --><partinfo>BBa_J52008 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_J52008 EndReviews</partinfo>

Latest revision as of 13:31, 30 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J52008

User Reviews

UNIQd5568e1e1a617367-partinfo-00000000-QINU UNIQd5568e1e1a617367-partinfo-00000001-QINU

•••••

iGEM TUDelft 2008 Ostassen

During our project of constructing an RNA thermometer to repress RNA translation we've used this part succesfully to produce luciferase. For more info check [http://2008.igem.org/Team:TUDelft/Temperature_results#Luciferase_Measurements this link]. Graphs visible over there are luciferase activity measurements.

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•••••

Catramp

The Utah_State 2011 iGEM Team has confirmed that this part emits light when placed under control of a promoter and the appropriate luciferin is added, and is capable of working with standard luciferase testing kits and luminometers.

Improvements: This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier (Dual Luciferase Assay Component).

;
•••••

UT-Tokyo

UT-Tokyo 2011 Team has characterized that this part emits luminescence with a promoter and substrate (D-luciferin). For testing this device, we carried out a promoter assay using BBa_R0011 and BBa_J23119 as a control. We succesfully evaluated the relative expression levels of BBa_R0011 with various IPTG concentration, compared to that of BBa_J23119. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].

Improvements: UT-Tokyo 2011 team has utilized this part to devise a Firefly-Renilla Dual Luciferase Assay Kit. This kit makes an evaluation of promoters and other cis-elements much easyer and more quantitative than existing devices (including GFP-reporter system). We also conducted a calibration of Photinus pyralis luciferase using a standard reagent, demonstrating that intracellular luciferase amount can be directly estimated from relative luminescence unit (RPU) by a linear regression. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].

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UNIQd5568e1e1a617367-partinfo-00000005-QINU