Difference between revisions of "Part:BBa K566015"
| (One intermediate revision by one other user not shown) | |||
| Line 4: | Line 4: | ||
Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required. | Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required. | ||
| − | A RBS sequence from the Elowitz repressilator that defines the RBS efficiency (<bbpart>BBa_B0034</bbpart>. Also contain the cI434 coding sequence optimized for ''E. coli'', its come from the registered part cI434 repressor protein (<bbpart>BBa_C0052</bbpart>), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation. | + | A RBS sequence from the Elowitz repressilator that defines the RBS efficiency (<bbpart>BBa_B0034</bbpart>). Also contain the cI434 coding sequence optimized for ''E. coli'', its come from the registered part cI434 repressor protein (<bbpart>BBa_C0052</bbpart>), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | .. | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 02:03, 5 October 2011
pRM-RBS-cI434
Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.
A RBS sequence from the Elowitz repressilator that defines the RBS efficiency (BBa_B0034). Also contain the cI434 coding sequence optimized for E. coli, its come from the registered part cI434 repressor protein (BBa_C0052), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 547
Illegal AgeI site found at 558 - 1000COMPATIBLE WITH RFC[1000]