Difference between revisions of "Part:BBa K566015"
 
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<partinfo>BBa_K566015 short</partinfo>
 
<partinfo>BBa_K566015 short</partinfo>
  
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Composite part for Biphasic switch it needs to be coupe to BBa K566016 part.
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Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.  
  
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A RBS sequence from the Elowitz repressilator that defines the RBS efficiency (<bbpart>BBa_B0034</bbpart>). Also contain the cI434 coding sequence optimized for ''E. coli'', its come from the registered part cI434 repressor protein (<bbpart>BBa_C0052</bbpart>), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.
  
  
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K566015 parameters</partinfo>
 
<partinfo>BBa_K566015 parameters</partinfo>
 
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== References ==
 
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Michalowski CB, Short MD, Little JW (2004) Sequence tolerance of the phage λ PRM promoter: implications for evolution of gene regulatory circuitry <i>J Bacteriol</i> <b>186</b>:7899-7999.
 
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</body>
 
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Latest revision as of 02:03, 5 October 2011

pRM-RBS-cI434

Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.

A RBS sequence from the Elowitz repressilator that defines the RBS efficiency (BBa_B0034). Also contain the cI434 coding sequence optimized for E. coli, its come from the registered part cI434 repressor protein (BBa_C0052), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 547
    Illegal AgeI site found at 558
  • 1000
    COMPATIBLE WITH RFC[1000]