Difference between revisions of "Part:BBa K608003"
 
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<partinfo>BBa_K608003 short</partinfo>
 
<partinfo>BBa_K608003 short</partinfo>
  
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strong Promotor , medium RBS
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This part consists of a strong promoter combined with a medium RBS.
 
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<br/>
  
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you can insert your gene of interest behind this part.<br/>
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You can insert your gene of interest behind this part.<br/>
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If you want a functional protein you need a promotor and a ribosome binding site<br/>
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If you want a functional protein you need a promotor and a ribosome binding site
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for a proper DNA to protein translation.<br/>
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for proper gene expression.<br/>
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K608003 parameters</partinfo>
 
<partinfo>BBa_K608003 parameters</partinfo>
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/4/43/T--Austin_Utexas--Low_significant_burden.png" style = "width:200px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 5.6 ± 1.5% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the <a href="https://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team.</a></p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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  </p>
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</body>
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</html>

Latest revision as of 00:12, 4 September 2020

strong Promoter , medium RBS

This part consists of a strong promoter combined with a medium RBS.

You can insert your gene of interest behind this part.
If you want a functional protein you need a promotor and a ribosome binding site for proper gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters



Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 5.6 ± 1.5%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.