Difference between revisions of "Part:BBa K510036:Design"
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<partinfo>BBa_K510036 short</partinfo> | <partinfo>BBa_K510036 short</partinfo> | ||
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<partinfo>BBa_K510036 SequenceAndFeatures</partinfo> | <partinfo>BBa_K510036 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| + | As for the different sequences used in our construction, here it is a brief description of each one: | ||
| + | - '''cI promoter''' ([https://parts.igem.org/Part:BBa_R0051 Part: BBa_R0051]): The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor [https://parts.igem.org/wiki/index.php/Part:BBa_C0051 BBa_C0051]. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator. | ||
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| + | - '''RybB asRNA''': Taken from Bouvier et al. (2008). The device includes a RBS followed by the RybB asRNA and a terminator. | ||
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===Source=== | ===Source=== | ||
| + | It's a composite part which comes from different genes forming the bassic flip-flop with anothers improvements. According to Design Notes, the main source was the Registry of Standard Biological Parts although also nbci database was used to the following parts: LacI and SspB. Besides Tag sequences (DAS4, asRNA and OmpN fusion) were obtained from each articles (see references). | ||
| + | ===References=== | ||
| + | - Timothy S. G., Charles R. C. & James J. C. (2000). '''Construction of a genetic toggle switch in ''Escherichia coli'''''. Nature 403, 339-342. | ||
| + | - McGinness, K.E., Baker, T.A., and Sauer, R.T. (2006). '''Engineering Controllable Protein Degradation'''. Molecular Cell 22, 701-707. | ||
| − | + | - Bouvier, M., Sharma, C.M., Mika, F., Nierhaus, K.H., and Vogel, J. (2008). '''Small RNA Binding to 5’ mRNA Coding Region Inhibits Translational Initiation'''. Molecular Cell 32, 827-837. | |
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| + | '''For further information please vistit our [http://2011.igem.org/Team:UPO-Sevilla wiki] or contact with us at igem@upo.es''' | ||
Latest revision as of 16:09, 21 September 2011
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Status: 500 Content-type: text/html
Software error:
Can't find string terminator '"' anywhere before EOF at /websites/parts.igem.org/cgi/lib/Range.pm line 311. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Design Notes
As for the different sequences used in our construction, here it is a brief description of each one:
- cI promoter (Part: BBa_R0051): The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator.
- RybB asRNA: Taken from Bouvier et al. (2008). The device includes a RBS followed by the RybB asRNA and a terminator.
Source
It's a composite part which comes from different genes forming the bassic flip-flop with anothers improvements. According to Design Notes, the main source was the Registry of Standard Biological Parts although also nbci database was used to the following parts: LacI and SspB. Besides Tag sequences (DAS4, asRNA and OmpN fusion) were obtained from each articles (see references).
References
- Timothy S. G., Charles R. C. & James J. C. (2000). Construction of a genetic toggle switch in Escherichia coli. Nature 403, 339-342.
- McGinness, K.E., Baker, T.A., and Sauer, R.T. (2006). Engineering Controllable Protein Degradation. Molecular Cell 22, 701-707.
- Bouvier, M., Sharma, C.M., Mika, F., Nierhaus, K.H., and Vogel, J. (2008). Small RNA Binding to 5’ mRNA Coding Region Inhibits Translational Initiation. Molecular Cell 32, 827-837.
For further information please vistit our [http://2011.igem.org/Team:UPO-Sevilla wiki] or contact with us at igem@upo.es