Difference between revisions of "Part:BBa K660003"

Latest revision as of 13:34, 21 September 2011

iLOV + RBS + TT

A composite part featuring the iLOV biobrick (https://parts.igem.org/Part:BBa_K660004) with an RBS (https://parts.igem.org/Part:BBa_B0030) and double terminator (https://parts.igem.org/Part:BBa_B1006).

iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. iLOV has the same function and uses as LOV2, but contains mutations that caused increased intensity in brightness.

As a reporter, it is advantageous over GFP derived fluorescent proteins for a number of reasons. Firstly, it is small in size so can be used when tagging proteins. It also recovers quickly from photobleaching. Uniquely, it has the ability to function in anoxic conditions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K660003 short</partinfo>
-A composite part featuring the iLOV biobrick (K660004) with an RBS (B0030) and double terminator (B1006)
+A composite part featuring the iLOV biobrick (https://parts.igem.org/Part:BBa_K660004) with an RBS (https://parts.igem.org/Part:BBa_B0030) and double terminator (https://parts.igem.org/Part:BBa_B1006).
+iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. iLOV has the same function and uses as LOV2, but contains mutations that caused increased intensity in brightness.
+As a reporter, it is advantageous over GFP derived fluorescent proteins for a number of reasons. Firstly, it is small in size so can be used when tagging proteins. It also recovers quickly from photobleaching. Uniquely, it has the ability to function in anoxic conditions.
 <!-- Add more about the biology of this part here