Difference between revisions of "Part:BBa K523023"
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An unexpected deletion of 9 bases was present in the 2nd spacer. The sequence entered takes this into account. | An unexpected deletion of 9 bases was present in the 2nd spacer. The sequence entered takes this into account. | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K523023 SequenceAndFeatures</partinfo> | <partinfo>BBa_K523023 SequenceAndFeatures</partinfo> | ||
− | + | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K523023 parameters</partinfo> | <partinfo>BBa_K523023 parameters</partinfo> | ||
− | < | + | |
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+ | ==Functional Parameters: Austin_UTexas== | ||
+ | <html> | ||
+ | <body> | ||
+ | <h3><center>Burden Imposed by this Part:</center></h3> | ||
+ | <figure> | ||
+ | <div class = "center"> | ||
+ | <center><img src = "https://static.igem.org/mediawiki/parts/4/43/T--Austin_Utexas--Low_significant_burden.png" style = "width:200px;height:120px"></center> | ||
+ | </div> | ||
+ | <figcaption><center><b>Burden Value: 9.8 ± 3.9% </b></center></figcaption> | ||
+ | </figure> | ||
+ | <p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the <a href="https://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team.</a></p> | ||
+ | <p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p> | ||
+ | </p> | ||
+ | </body> | ||
+ | </html> |
Latest revision as of 00:28, 4 September 2020
PlacLacZ + EYFP
This is the lac promoter driving Enhanced Yellow Fluorescent Protein. It was made as a test of the BioSandwich protocol of Edinburgh 2011.
An unexpected deletion of 9 bases was present in the 2nd spacer. The sequence entered takes this into account.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.