Difference between revisions of "Part:BBa K523008"
 
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<partinfo>BBa_K523008 short</partinfo>
 
<partinfo>BBa_K523008 short</partinfo>
  
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This is a modified version of <partinfo>BBa_K265008</partinfo> for use with the BioSandwich protocol of Edinburgh 2011.
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This is a modified version of <partinfo>BBa_K265008</partinfo> for use with the BioSandwich protocol (<nowiki>RFC 81</nowiki>) of Edinburgh 2011.
  
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BioSandwich assembly requires an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
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When used to make fusion proteins, BioSandwich assembly requires an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end of the BioBrick. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
  
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Incidentally, although this part has been made in RFC10 format, the two extra bases should mean that joining it to an RFC23 part would create a viable fusion.
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Incidentally, although this part has been made in RFC10 format, the two extra bases might help in some fusion applications using other protocols.
  
 
===Usage and Biology===
 
===Usage and Biology===
  
 
The DNA is a codon-optimised version of the N and C terminal domains of ''Pseudomonas syringae'' Ice Nucleation Protein. It has been shown that, when INPNC is fused to another protein (the "passenger"), it causes the passenger to be displayed on the outer membrane of ''E. coli''.
 
The DNA is a codon-optimised version of the N and C terminal domains of ''Pseudomonas syringae'' Ice Nucleation Protein. It has been shown that, when INPNC is fused to another protein (the "passenger"), it causes the passenger to be displayed on the outer membrane of ''E. coli''.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K523008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K523008 SequenceAndFeatures</partinfo>
  

Latest revision as of 20:18, 18 September 2011

INP for BioSandwich

This is a modified version of BBa_K265008 for use with the BioSandwich protocol (RFC 81) of Edinburgh 2011.

When used to make fusion proteins, BioSandwich assembly requires an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end of the BioBrick. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

Incidentally, although this part has been made in RFC10 format, the two extra bases might help in some fusion applications using other protocols.

Usage and Biology

The DNA is a codon-optimised version of the N and C terminal domains of Pseudomonas syringae Ice Nucleation Protein. It has been shown that, when INPNC is fused to another protein (the "passenger"), it causes the passenger to be displayed on the outer membrane of E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]