Difference between revisions of "Part:BBa K404094"
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K404094 short</partinfo>
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<partinfo>BBa_K404094 short</partinfo><br />
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404094 [AAV2]-AAPex]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_K404094 BBa_K404094]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|pAAV_MCS provided by Stratagene
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|-
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|'''Submitted by'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
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|}
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<br /><br /><br /><br /><br /><br /><br /><br /><br><br><br><br>
  
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[[Image:Freiburg10_ReplicationBricks 5.png|thumb|center|480px]]<br>
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[[Image:Freiburg10_ReplicationBricks 5.png|thumb|left|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br>
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 03:34, 28 October 2010

[AAV2]-AAPex

[AAV2-AAPex]
BioBrick Nr. BBa_K404094
RFC standard RFC 10
Requirement pSB1C3
Source pAAV_MCS provided by Stratagene
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]














Freiburg10 ReplicationBricks 5.png













Usage and Biology

A gene encoding for the assembly-activating protein (AAP) was recently (in 2010) discovered in the Adeno-associated virus (AAV) serotype 2 genome. Its gene product is conserved among all AAV serotypes, illustrating its essential role in virus life cycle. Its functions comprise transport of the viral structural proteins to the nucleolus and involvement in following capsid assembly. The AAP gene, located in the Cap coding region, is translated from an alternative open reading frame (ORF) with unconventional start codon. If modifications need to be introduced in the AAV capsid – for example for targeting approaches – the AAP has to be taken in account in order to prevent virus assembly impairment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]