Difference between revisions of "Part:BBa K316004"

Latest revision as of 03:22, 28 October 2010

Functional XylE under J23101 promoter, with double terminator

Standard constitutive E.coli promoter BBa_J23101 combined with BBa_K316003 for comparative characterisation of gene activity (catechol breakdown into 2,4 hydromuconic semialdehyde yellow product).

For more information about XylE, it's substrate and spectrophotometric assays, please see BBa_K316003 or our [http://2010.igem.org/Team:Imperial_College_London/Results wiki results section]

Safety

The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the [http://www.sciencelab.com/msds.php?msdsId=9927131 Material Safety Data Sheet].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 380
Illegal NgoMIV site found at 552
Illegal AgeI site found at 903
1000
COMPATIBLE WITH RFC[1000]

Part Characterisation

Characterisation data was obtained for XylE BBa_K316003. In addition constructs under two different promoters: J23101-XylE BBa_K316004 from E. coli was used to categorise B. subtilis derived Pveg-XylE BBa_K316005. Also GFP-XylE constructs BBa_K316007 were tested to determine the effectiveness of inhibition of XylE activity by attachment of GFP. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K316004 short</partinfo>
-Constitutive E.coli promoter <bbpart>BBa_J23101</bbpart> combined with <bbpart>BBa_S04510</bbpart>for easy characterisation of gene activity (catechol breakdown into 2,4 hydroxyaminobutane yellow product) under a standard E.coli promoter
+Standard constitutive E.coli promoter <bbpart>BBa_J23101</bbpart> combined with <bbpart>BBa_K316003</bbpart> for comparative characterisation of gene activity (catechol breakdown into 2,4 hydromuconic semialdehyde yellow product).
+For more information about XylE, it's substrate and spectrophotometric assays, please see <bbpart>BBa_K316003</bbpart> or our [http://2010.igem.org/Team:Imperial_College_London/Results wiki results section]
+'''Safety'''
+The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the [http://www.sciencelab.com/msds.php?msdsId=9927131 Material Safety Data Sheet].
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 <partinfo>BBa_K316004 SequenceAndFeatures</partinfo>
@@ Line 17: / Line 26: @@
 <partinfo>BBa_K316004 parameters</partinfo>
 <!-- -->
+<span class='h3bb'><big>'''Part Characterisation'''</big></span>
+----
+Characterisation data was obtained for XylE <bbpart>BBa_K316003</bbpart>. In addition constructs under two different promoters: J23101-XylE <bbpart>BBa_K316004</bbpart> from ''E. coli'' was used to categorise ''B. subtilis'' derived Pveg-XylE <bbpart>BBa_K316005</bbpart>. Also GFP-XylE constructs <bbpart>BBa_K316007</bbpart> were tested to determine the effectiveness of inhibition of XylE activity by attachment of GFP. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.