Difference between revisions of "Part:BBa K404213"

Latest revision as of 02:43, 28 October 2010

ViralBrick-587KO-Z34C

pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)
BioBrick Nr.	BBa_K404165
RFC standard	RFC 10
Requirement	pSB1C3
Source
Submitted by	[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop

Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Restriction sites

Restriction site	Enzyme	Recognition sequence	Activity	Temp.
upstream 587	[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]		100;50;10;100	37°C
downstream 587	[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]		0;25;0;100	37°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 10
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K404213 short</partinfo>
-{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
-! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404213 ViralBrick-587KO-Z34C]
-|-
-|'''BioBrick Nr.'''
-|[https://parts.igem.org/Part:BBa_K404213 BBa_K404213]
-|-
-|'''RFC standard'''
-|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
-|-
-|'''Requirement'''
-|pSB1C3<br>
-|-
-|'''Source'''
-|
-|-
-|'''Submitted by'''
-|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
-|}
-<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
 ! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404165 pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)]