Difference between revisions of "Part:BBa K389016"
(→Important parameters) |
(→Important parameters) |
||
| Line 20: | Line 20: | ||
!Value | !Value | ||
|- | |- | ||
| − | |rowspan="4"|[[Part:BBa_K389016:Experience#Transfer function of BBa_K389016 | Transfer | + | |rowspan="4"|[[Part:BBa_K389016:Experience#Transfer function of BBa_K389016 | Transfer function]] |
|Maximum induction level | |Maximum induction level | ||
|2.6 fold | |2.6 fold | ||
| Line 60: | Line 60: | ||
|Acetosyringone | |Acetosyringone | ||
|- | |- | ||
| − | |rowspan="4"|No | + | |rowspan="4"|No induction by |
|Capsaicin | |Capsaicin | ||
|- | |- | ||
Latest revision as of 00:17, 28 October 2010
VirA/G reporter device mRFP
This BioBrick contains a complete VirA/G receptor system with an mRFP (BBa_E1010) under the control of a vir promoter as reporter gene.
Input: acetosyringone
Output: expression of mRFP
Usage and Biology
This BioBrick is for testing, characterizing and measuring the VirA/G signaling system. This system contains an unmutated virA, so it is possible to measure how the natural VirA/G system works and reacts. It is also possible to determine the basal transcription of the vir promoter and its activity after inducing the system with acetosyringone.
Important parameters
| Experiment | Characteristic | Value |
|---|---|---|
| Transfer function | Maximum induction level | 2.6 fold |
| Maximum induction level reached | 150 µM acetosyringone | |
| Hill coefficient | 1.67 | |
| Switch Point | 26.5 µM acetosyringone | |
| Doubling time / h | without plasmid | 1.98 |
| carrying K389016 | 2.57 | |
| carrying K389016 with 150 µM acetosyringone | 2.77 | |
| carrying K389016 with 1000 µM acetosyringone | 3.01 | |
| Conformation analysis | ratio ccc monomer / % | 91.2 |
| ratio ccc dimer / % | 3.2 | |
| ratio oc forms / % | 5.6 | |
| Inducers | Induction by | Acetosyringone |
| No induction by | Capsaicin | |
| Dopamine | ||
| Homovanillic acid | ||
| 3-Methoxytyramine |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4260
Illegal AgeI site found at 4372 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768