Difference between revisions of "Part:BBa K316019"

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<partinfo>BBa_K316019 short</partinfo>
 
<partinfo>BBa_K316019 short</partinfo>
  
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ComE binds to a specific sequence leading to activation of transcription. This part is the reverse of the BioBrick <bbpart>BBa_K316018</bbpart>.
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This part is the 3' coding version ofthe BioBrick <bbpart>BBa_K316018</bbpart>. ComE <bbpart>BBa_K316016</bbpart> binds to a specific sequence in a promoter leading to activation of transcription downstream of the binding site. This sequence regulates the ComE responsive promoter in the ComCDE operon. This sequence has been previously shown to be sufficient to drive ComE regulated transcription within ''S. pneumoniae''[http://www.ncbi.nlm.nih.gov/pubmed/17328748].
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[[Image:ComEbs.PNG|center|500px]]
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'''Figure I.''' ComE binding sites in ''S. pneumonia'' genome. Top - ComAB operon, Middle - ComCDE operon, Bottom - consensus sequence. Source[http://www.ncbi.nlm.nih.gov/pubmed/15126469]
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For more information about ComCDE system, please check our wiki [http://2010.igem.org/Team:Imperial_College_London/Modules/Signaling]
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K316019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K316019 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K316019 parameters</partinfo>
 
<partinfo>BBa_K316019 parameters</partinfo>
 
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===References===
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<biblio>
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#1 A new integrative reporter plasmid for Streptococcus pneumoniae. Halfmann et al (2007)[http://www.ncbi.nlm.nih.gov/pubmed/17328748]
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</biblio>

Latest revision as of 23:53, 27 October 2010

Reverse strand coding ComE responsive promoter

This part is the 3' coding version ofthe BioBrick BBa_K316018. ComE BBa_K316016 binds to a specific sequence in a promoter leading to activation of transcription downstream of the binding site. This sequence regulates the ComE responsive promoter in the ComCDE operon. This sequence has been previously shown to be sufficient to drive ComE regulated transcription within S. pneumoniae[http://www.ncbi.nlm.nih.gov/pubmed/17328748].


ComEbs.PNG

Figure I. ComE binding sites in S. pneumonia genome. Top - ComAB operon, Middle - ComCDE operon, Bottom - consensus sequence. Source[http://www.ncbi.nlm.nih.gov/pubmed/15126469]

For more information about ComCDE system, please check our wiki [http://2010.igem.org/Team:Imperial_College_London/Modules/Signaling]



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<biblio>

  1. 1 A new integrative reporter plasmid for Streptococcus pneumoniae. Halfmann et al (2007)[http://www.ncbi.nlm.nih.gov/pubmed/17328748]

</biblio>