Difference between revisions of "Part:BBa K325909:ArabinosetoLight"
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==Data==
 
==Data==
  
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[[Image:BBa_K325909Aracurve.png|thumb|569px|center|'''Figure 1 - Light output of <partinfo>K325219</partinfo> as a function of Arabinose concentration in the media. The values correspond to the peak intensity within 5 hours of adding Arabinose to the media. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats.  ''']]
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[[Image:BBa_K325909Aracurve.png|thumb|569px|center|'''Figure 1 - Light output of <partinfo>K325909</partinfo> as a function of Arabinose concentration in the media. The values correspond to the peak intensity within 5 hours of adding Arabinose to the media. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats.  ''']]
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[[Image:BBa_K325909timecourse.png|thumb|569px|center|'''Figure 2 - Evolution of luminescence with time at different Arabinose concentrations for <partinfo>K325219</partinfo>. Measurements were taken every 30 minutes. Data points and error bars correspond to the mean and standard deviation of 3 time repeats. ''']]
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[[Image:BBa_K325909timecourse.png|thumb|569px|center|'''Figure 2 - Evolution of luminescence with time at different Arabinose concentrations for <partinfo>K325909</partinfo>. Measurements were taken every 30 minutes. Data points and error bars correspond to the mean and standard deviation of 3 time repeats. ''']]
 
<center>
 
<center>
 
{|{{Table}}
 
{|{{Table}}
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==Protocol==
 
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#The protocol for
 
  
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<math>
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P_{out} = \frac{P_{max}[3OC_6HSL]^n}{K^n+[3OC_6HSL]^n}
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</math>
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</div>
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</center>
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<sup>1</sup>
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</div>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
 
</div>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
 
<div style="padding: 00px; width: 680px">
 
<div style="padding: 00px; width: 680px">
 
'''Compatibility'''<br>
 
'''Compatibility'''<br>
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br>
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)'', [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan] and H-NS mutant JM 230 H-NS -205::tn10.<br>
 
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
 
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
  

Latest revision as of 21:21, 27 October 2010

Input: L-Arabinose
Output: Light

pBad/araC
I0500		PoPS to Light
 Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       


Description
This page describes the light output of BBa K325909 as a fucntion of Arabinose concentration in the media. The characterisation has been made in different chassis. TOP 10 cells as well as 2 mutants in which expression of H-NS was repressed.


Data

Figure 1 - Light output of BBa_K325909 as a function of Arabinose concentration in the media. The values correspond to the peak intensity within 5 hours of adding Arabinose to the media. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats.
Figure 2 - Evolution of luminescence with time at different Arabinose concentrations for BBa_K325909. Measurements were taken every 30 minutes. Data points and error bars correspond to the mean and standard deviation of 3 time repeats.
Data Notes Date Uploaded
Media:BBa_K325909ArabinosetoLight.xls Raw data from experiment 21/10/2010


Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen), [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan] and H-NS mutant JM 230 H-NS -205::tn10.
Plasmids: Device has been shown to work on pSB1C3


References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176. [http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.