Difference between revisions of "Part:BBa K331030:Experience"

Revision as of 20:09, 27 October 2010

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Applications of BBa_K331030

In order to further characterize the C-terminal and N-terminal oligoarginine tag (BioBricks BBa_K249005 and BBa_K249004 respectively) and investigate the effect their placement on protein stability, yellow fluorescent proteins (YFP) with the oligoarginine fused to either the C-terminus (BBa_K331023) or N-terminus (BBa_K331022) (and preceded by a ribosomal binding site – BBa_B0034) were synthesized. We used our Red/White 3-Antibiotic assembly method to add a tetracycline repressible promoter (BBa_R0040) for constitutive expression of the fusion protein. This addition generated BioBricks BBa_K331031 and BBa_K331030 for the C-terminal tagged and N-terminal tagged YFP respectively.

User Reviews

UNIQ92771b48b4d61274-partinfo-00000009-QINU UNIQ92771b48b4d61274-partinfo-0000000A-QINU

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 ===Applications of BBa_K331030===
+In order to further characterize the C-terminal and N-terminal oligoarginine tag (BioBricks <html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K249005" target="new"><font color="#00DC00">BBa_K249005</font></a></html> and <html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K249004" target="new"><font color="#00DC00">BBa_K249004</font></a></html> respectively) and investigate the effect their placement on protein stability, yellow fluorescent proteins (YFP) with the oligoarginine fused to either the C-terminus (<html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K331023" target="new"><font color="#00DC00">BBa_K331023</font></a></html>) or N-terminus (<html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K331022" target="new"><font color="#00DC00">BBa_K331022</font></a></html>) (and preceded by a ribosomal binding site – <html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_B0034" target="new"><font color="#00DC00">BBa_B0034</font></a></html>) were synthesized. We used our <html><a href="http://2010.igem.org/Team:Lethbridge/Notebook/Protocols#Assembly_of_BioBricks_using_the_Red.2FWhite_3-Antibiotic_Assembly_Method"><font color="#00DC00"> Red/White 3-Antibiotic assembly method</font></a></html> to add a tetracycline repressible promoter (<html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_R0010" target="new"><font color="#00DC00">BBa_R0040</font></a></html>) for constitutive expression of the fusion protein. This addition generated BioBricks <html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K331031" target="new"><font color="#00DC00">BBa_K331031</font></a></html> and <html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K331030" target="new"><font color="#00DC00">BBa_K331030</font></a></html> for the C-terminal tagged and N-terminal tagged YFP respectively.
 ===User Reviews===