Difference between revisions of "Part:BBa K316004"
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− | Characterisation data was obtained using GFP-XylE constructs <bbpart> | + | Characterisation data was obtained using GFP-XylE constructs <bbpart>BBa_K316007</bbpart> and XylE under two different promoters: ''B. subtilis'' derived Pveg <bbpart>BBa_K316005</bbpart> and J23101 <bbpart>BBa_K316004</bbpart> from ''E. coli''. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages. |
Revision as of 10:43, 27 October 2010
Functional XylE under J23101 promoter, with double terminator
Standard constitutive E.coli promoter BBa_J23101 combined with BBa_K316003 for comparative characterisation of gene activity (catechol breakdown into 2,4 hydroxyaminobutane yellow product).
For more information about XylE, it's substrate and spectrophotometric assays, please see BBa_K316003 or our wiki[http://2010.igem.org/Team:Imperial_College_London/Results]
Safety
The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the Material Safety Data Sheet[http://www.sciencelab.com/msds.php?msdsId=9927131].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 380
Illegal NgoMIV site found at 552
Illegal AgeI site found at 903 - 1000COMPATIBLE WITH RFC[1000]
Part Characterisation
Characterisation data was obtained using GFP-XylE constructs BBa_K316007 and XylE under two different promoters: B. subtilis derived Pveg BBa_K316005 and J23101 BBa_K316004 from E. coli. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.