Difference between revisions of "Part:BBa K316004"
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Standard constitutive E.coli promoter <bbpart>BBa_J23101</bbpart> combined with <bbpart>BBa_K316003</bbpart> for comparative characterisation of gene activity (catechol breakdown into 2,4 hydroxyaminobutane yellow product).
 
Standard constitutive E.coli promoter <bbpart>BBa_J23101</bbpart> combined with <bbpart>BBa_K316003</bbpart> for comparative characterisation of gene activity (catechol breakdown into 2,4 hydroxyaminobutane yellow product).
  
 +
For more information about XylE, it's substrate and spectrophotometric assays, please see <bbpart>BBa_K316003</bbpart> or our wiki[http://2010.igem.org/Team:Imperial_College_London/Results]
  
 
'''Safety'''
 
'''Safety'''

Revision as of 09:35, 27 October 2010

Functional XylE under J23101 promoter, with double terminator

Standard constitutive E.coli promoter BBa_J23101 combined with BBa_K316003 for comparative characterisation of gene activity (catechol breakdown into 2,4 hydroxyaminobutane yellow product).

For more information about XylE, it's substrate and spectrophotometric assays, please see BBa_K316003 or our wiki[http://2010.igem.org/Team:Imperial_College_London/Results]

Safety

The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the Material Safety Data Sheet[http://www.sciencelab.com/msds.php?msdsId=9927131].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal NgoMIV site found at 552
    Illegal AgeI site found at 903
  • 1000
    COMPATIBLE WITH RFC[1000]


Part Characterisation

Characterisation data was obtained using GFP-XylE constructs BBa_K316008 and XylE under two different promoters: B. subtilis derived Pveg BBa_K316005 and J23101 BBa_K316004 from E. coli. These are described in our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the relevant parts pages.


References

<biblio>

  1. 1

</biblio>