Difference between revisions of "Part:BBa K415031"

(Removal of Misplaced Data)
 
(4 intermediate revisions by the same user not shown)
Line 4: Line 4:
 
This part is a promoter that was designed as a correction to the anomalous [[Part:BBa_R0065]] BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.
 
This part is a promoter that was designed as a correction to the anomalous [[Part:BBa_R0065]] BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.
  
−
Slightly stronger binding than [[Part:BBa_K415032]].
+
Slightly stronger binding than [[Part:BBa_K415032]]. For proof of reduced leakiness, see [[Part:BBa_K415032]].
  
−
{|style="text-align: center; border: 1px solid black;"
+
<!-- Add more about the biology of this part here
−
|-
+
===Usage and Biology===
−
!
+
 
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K415031 SequenceAndFeatures</partinfo>
 +
 
 +
 
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K415031 parameters</partinfo>
 +
<!-- -->

Latest revision as of 06:21, 27 October 2010

Improved PLux/cI-OR Promoter

This part is a promoter that was designed as a correction to the anomalous Part:BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.

Slightly stronger binding than Part:BBa_K415032. For proof of reduced leakiness, see Part:BBa_K415032.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]