Difference between revisions of "Part:BBa K316005"
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<partinfo>BBa_K316005 parameters</partinfo>
 
<partinfo>BBa_K316005 parameters</partinfo>
 
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<span class='h3bb'><big>'''Part Characterisation'''</big></span>
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Characterisation data was obtained using GFP-XylE constructs <bbpart>BBa_K316008</bbpart> and XylE under two different promoters: ''B. subtilis'' derived Pveg <bbpart>BBa_K316005</bbpart> and J23101 <bbpart>BBa_K316004</bbpart> from ''E. coli''. These are described in our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the relevant parts pages.

Revision as of 22:26, 26 October 2010

Functional XylE under Pveg promoter, with double terminator

Enables characterization and comparison with alternative promoters e.g. BBa_J23101 Designed for characterisation of XylE in Bacillus subtilis where Pveg is a useful constitutive promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 442
    Illegal NgoMIV site found at 614
    Illegal AgeI site found at 965
  • 1000
    COMPATIBLE WITH RFC[1000]


Part Characterisation

Characterisation data was obtained using GFP-XylE constructs BBa_K316008 and XylE under two different promoters: B. subtilis derived Pveg BBa_K316005 and J23101 BBa_K316004 from E. coli. These are described in our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the relevant parts pages.