Difference between revisions of "Part:BBa K316007"
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<partinfo>BBa_K316007 short</partinfo>
 
<partinfo>BBa_K316007 short</partinfo>
  
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This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. This construct does not have a terminator at the end, another construct <bbpart>BBa_K143008</bbpart> contains a double terminator <bbpart>BBa_B0014</bbpart>.
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This construct contains <bbpart>BBa_K316006</bbpart> under the control of Pveg promoter <bbpart>BBa_K143053</bbpart>. It is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. This construct does not have a terminator at the end, another construct <bbpart>BBa_K143008</bbpart> contains a double terminator <bbpart>BBa_B0014</bbpart>.
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===Structure and Features===
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[[Image:GFPXylE.PNG|center|800px]]
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'''Figure I.''' Graphical representation of the GFP-XylE construct with associated Pveg promoter, tags and linkers.
  
  
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K316007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K316007 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K316007 parameters</partinfo>
 
<partinfo>BBa_K316007 parameters</partinfo>
 
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For more information about our project please visit our wiki [http://2010.igem.org/Team:Imperial_College_London].

Revision as of 04:07, 26 October 2010

Recombinant GFP-XylE protein under Pveg promoter

This construct contains BBa_K316006 under the control of Pveg promoter BBa_K143053. It is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. This construct does not have a terminator at the end, another construct BBa_K143008 contains a double terminator BBa_B0014.


Structure and Features

GFPXylE.PNG

Figure I. Graphical representation of the GFP-XylE construct with associated Pveg promoter, tags and linkers.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1229
    Illegal NgoMIV site found at 1401
    Illegal AgeI site found at 1752
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 785



For more information about our project please visit our wiki [http://2010.igem.org/Team:Imperial_College_London].