Difference between revisions of "Part:BBa K316006"
(→Structure and Features) |
(→Structure and Features) |
||
Line 13: | Line 13: | ||
[[Image:naGFPXylE.PNG|center|800px]] | [[Image:naGFPXylE.PNG|center|800px]] | ||
+ | |||
+ | |||
+ | '''Figure I.''' Graphical representation of the GFP-XylE construct with associated tags and linkers. | ||
Revision as of 03:58, 26 October 2010
N-terminus his tagged-GFP-XylE fusion protein
Constructed to be combined with promoter and terminator. The GFP BBa_E0040 has a 5'(5xHis) tag and is linked to XylE BBa_J33204 monomer subunit (It should be noted that the RBS of XylE (J33204) has been removed for this fusion protein). The linker is composed of a Flag tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS - flexible linker. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.
Parts were assembled by PCR primer extension for exact methods, see our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy]
Structure and Features
Figure I. Graphical representation of the GFP-XylE construct with associated tags and linkers.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1106
Illegal NgoMIV site found at 1278
Illegal AgeI site found at 1629 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662
For more information about our project please visit our wiki [http://2010.igem.org/Team:Imperial_College_London].