Difference between revisions of "Part:BBa K342002:Design"
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<a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font> | <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font> | ||
</a></p> | </a></p> | ||
− | <p>The phasin sequence is the same as the biobrick <a | + | <p>The phasin sequence is the same as the biobrick <a href="https://parts.igem.org/Part:BBa_K208001"><font color= "black"><i><u>BBa_K208001</u></i></font></a> from the Utah 2009 team. |
<p>The sequencing of this part is difficult because of the repetition of phasin's sequence.</p> | <p>The sequencing of this part is difficult because of the repetition of phasin's sequence.</p> | ||
</html> | </html> |
Revision as of 14:14, 25 October 2010
Phasin-Phasin Intein (for silver standard fusion)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1693
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1279
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been designed thanks to the results of the publication Banki et al.
The phasin sequence is the same as the biobrick BBa_K208001 from the Utah 2009 team.
The sequencing of this part is difficult because of the repetition of phasin's sequence.
Source
Ligation of two synthetized parts : Phasin and Phasin-Intein