Difference between revisions of "Part:BBa K404117"
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__NOTOC__
 
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<partinfo>BBa_K404117 short</partinfo>
 
<partinfo>BBa_K404117 short</partinfo>
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[[Image:Freiburg10_Vectorplasmid precursors 4.png|thumb|center|480px]]<br>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404201 ViralBrick-453-BAP]
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404117 [AAV2]-left-ITR_pCMV_betaglobin]
 
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! colspan="2"|[[Image:Freiburg10_Vectorplasmid precursors 4.png|200px]]
 
! colspan="2"|[[Image:Freiburg10_Vectorplasmid precursors 4.png|200px]]
 
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|'''BioBrick Nr.'''
 
|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_K404117 BBa_K404117]
 
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|'''RFC standard'''
 
|'''RFC standard'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
 
|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
 
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Constructing viral particles encapsidating the transgene expression cassette flanked by the inverted terminal repeats (ITRs, BBa_K404100 and BBa_K404101) can be used in several applications, such as efficient tumor killing or quantification assays. <br />
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The plasmid containing the desired gene of interest which is cotransfected with the RepCap plasmid (BBa_K404001, BBa_K404002 or BBa_K404003) and the pHelper plasmid. Several assembly steps have to be performed in order to obtain the fully assembled vectorplasmid.  Facilitating the cloning steps the iGEM team Freiburg_Bioware 2010 provide the 3' nucleotide components in respect to the desired gene of interest which are the left inverted terminal repeat (BBa_K404100) followed by the CMV promoter (BBa_K404102) and a putative enhancer-element (BBa_K404107).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 17:19, 20 October 2010

[AAV2]-left-ITR_pCMV_betaglobin

[AAV2-left-ITR_pCMV_betaglobin]
Freiburg10 Vectorplasmid precursors 4.png
BioBrick Nr. BBa_K404117
RFC standard RFC 10
Requirement pSB1C3
Source synthetic
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]


Constructing viral particles encapsidating the transgene expression cassette flanked by the inverted terminal repeats (ITRs, BBa_K404100 and BBa_K404101) can be used in several applications, such as efficient tumor killing or quantification assays.
The plasmid containing the desired gene of interest which is cotransfected with the RepCap plasmid (BBa_K404001, BBa_K404002 or BBa_K404003) and the pHelper plasmid. Several assembly steps have to be performed in order to obtain the fully assembled vectorplasmid. Facilitating the cloning steps the iGEM team Freiburg_Bioware 2010 provide the 3' nucleotide components in respect to the desired gene of interest which are the left inverted terminal repeat (BBa_K404100) followed by the CMV promoter (BBa_K404102) and a putative enhancer-element (BBa_K404107).








Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]