Difference between revisions of "Part:BBa K329003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K329003 short</partinfo> | <partinfo>BBa_K329003 short</partinfo> | ||
| − | Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Strong RBS upstream (<partinfo>BBa_B0034</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see <partinfo>BBa_K329000</partinfo>) | + | Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Strong RBS upstream (<partinfo>BBa_B0034</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. |
| + | <br>We have designed several translational units for TetA(C) (see <partinfo>BBa_K329000</partinfo>, <partinfo>BBa_K329004</partinfo>). | ||
Latest revision as of 16:31, 12 October 2010
Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)
Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Strong RBS upstream (BBa_B0034). However, overexpression of TetA(C) is detrimental to cell growth.
We have designed several translational units for TetA(C) (see BBa_K329000, BBa_K329004).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 162
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 308
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 334
Illegal NgoMIV site found at 702
Illegal NgoMIV site found at 862 - 1000COMPATIBLE WITH RFC[1000]