Difference between revisions of "Part:BBa K389010:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K389010 short</partinfo>
 
<partinfo>BBa_K389010 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The ''virA'' gene is under the control a medium strong constitutive promotor.
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The ''virA'' gene is under the control a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)
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===Source===
 
===Source===
  
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''A. tumefaciens'' C58, parts.igem
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* ''virA'' from ''A. tumefaciens'' C58 (<partinfo>K389001</partinfo>)
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* constitutive promoter and RBS from parts.igem (<partinfo>J23110</partinfo>, <partinfo>B0034</partinfo>)
  
 
===References===
 
===References===

Latest revision as of 12:08, 7 October 2010

VirA receptor (expressed)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768


Design Notes

The virA gene is under the control a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)

Source

References