Difference between revisions of "Part:pSB1AC0"

 
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<partinfo>pSB1AC0 short</partinfo>
 
<partinfo>pSB1AC0 short</partinfo>
  
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This part is derived from pSB1AC3-P1010.  pSB1AC3-P1010 was cut with NotI (to remove the insert) and then religated together.  Thus, pSB1AC0 is the empty plasmid in which the BioBricks cloning site has been partially destroyed.  The EcoRI and PstI sites remain.  This empty plasmid is a useful control in characterization experiments because it serves as a baseline for cell morphology, fluorescence, growth etc. in the presence of plasmid but in the absence of any insert.
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This part is derived from pSB1AC3-P1010.  pSB1AC3-P1010 was cut with NotI (to remove the insert) and then religated together.  Thus, pSB1AC0 is the empty plasmid in which the BioBricks cloning site has been partially destroyed.  The EcoRI and PstI sites remain.  This empty plasmid is a useful control in characterization experiments.  This plasmid serves as a point of comparison to constructs of interest.  Hence effects on morphology, fluorescence, growth rate etc. by the plasmid itself can be accounted for.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:02, 1 June 2006

pSB1AC0

This part is derived from pSB1AC3-P1010. pSB1AC3-P1010 was cut with NotI (to remove the insert) and then religated together. Thus, pSB1AC0 is the empty plasmid in which the BioBricks cloning site has been partially destroyed. The EcoRI and PstI sites remain. This empty plasmid is a useful control in characterization experiments. This plasmid serves as a point of comparison to constructs of interest. Hence effects on morphology, fluorescence, growth rate etc. by the plasmid itself can be accounted for.

Usage and Biology

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3026
    Illegal PstI site found at 8
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3026
    Illegal PstI site found at 8
    Illegal NotI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3026
    Illegal XhoI site found at 1027
    Illegal XhoI site found at 1919
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3026
    Illegal PstI site found at 8
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3026
    Illegal PstI site found at 8
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2065

Functional Parameters