Difference between revisions of "Part:BBa J70628"
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<partinfo>BBa_J70628 short</partinfo> | <partinfo>BBa_J70628 short</partinfo> | ||
| + | This is a RFC12 compatible Intein self-splicing protein. It is capable of self cleavage induced by overnight incubation at 4°C with 50 mM DDT. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Intein domains are commonly fused with CBD (<partinfo>J70627</partinfo>) and then used to cleave off the CBD tag after purification, yielding very high levels of the target protein. Note that the target protein's N terminal or C terminal residue can affect cleavage. | ||
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Revision as of 20:42, 11 August 2010
RFC12 Sce VMA Intein Internal Domain This is a RFC12 compatible Intein self-splicing protein. It is capable of self cleavage induced by overnight incubation at 4°C with 50 mM DDT.
Usage and Biology
Intein domains are commonly fused with CBD (BBa_J70627) and then used to cleave off the CBD tag after purification, yielding very high levels of the target protein. Note that the target protein's N terminal or C terminal residue can affect cleavage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 486
Illegal BamHI site found at 1339 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1195
- 1000COMPATIBLE WITH RFC[1000]